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BACKGROUND: With a clear distinction from traditional computed tomography (CT) imaging with information absorption, phase-contrast CT with synchronous radiation has implemented the microstructure imaging of soft tissues in organisms with an unprecedented imaging mechanism. OBJECTIVE: To explore the synchrotron radiation phase-contrast CT imaging technology in the bone regeneration imaging after bone grafting. METHODS: Four New Zealand white rabbits were used to make a metaphyseal defect model. Then, model rabbits were randomized into a group with calcium phosphate bone grafting and a group with Bio-Oss bone grafting in the defects. The specimens were imaged by the synchrotron radiation phase-contrast CT and stained with hematoxylin-eosin and sirius red 2 weeks after bone grafting. RESULTS AND CONCLUSION: (1) Bio-Oss bone graft material group: Osteoid was observed not only around the graft material but also in the area far from the graft bone material as reticulate structure by the synchrotron radiation phase-contrast CT. Hematoxylin-eosin staining showed a large amount of red osteoid tissues arranged as trabecular bone, and a large amount of osteoblasts with obvious osteogensis. Sirius red-stained pathological sections were largely stained yellow, and there were round or oval osteoblasts with strongly expressed type I collagen. (2) Calcium phosphate bone graft material group: There was no reticulate structure shown by the synchrotron radiation phase-contrast CT, and the creep of osteoid tissues was only around the bone graft. Hematoxylin-eosin staining showed a large amount of red osteoid tissues, and sirius red-stained pathological sections were stained yellow and red. To conclude, the synchronous radiation phase-contrast CT can clearly display the regenerated structure of bone grafts.
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Through three treatment cases according to syndrome differentiation of intracoronary stenting postoperatively by warming the spleen and stomach for dispelling cold, replenishing qi to invigorate the spleen, eliminating phlegm and dredging channel blockade, invigorating vital energy, soothing the liver and regulating the spleen, and relieving mental stress, this article discussed the application of treatment according to syndrome differentiation in intracoronary stenting postoperatively.
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Objective To analyze the application of Downey classification and to improve the diagnosis and treatment for children bitten by venomous snakes.Methods The consecutive 76 snakebite children were treated according to the guidance of Downey classification in Children's Hospital of Chongqing Medical University from Jan.2005 to Dec.2011.Their epidemiological and clinical characteristics,treatment and outcomes were reviewed and analyzed.The Chinese traditional classification method was adopted to classify children of snakebites as a contrast at the same time,and the correlation of the 2 methods was studied.Correlations in Downey level,treatment time,length of hospital stay,osteofascial compartment syndrome (OCS) and the occurrence of myocardial damage were studied respectively.Results The 76 cases of snakebites all occurred from Apr.to Nov.,the time of injuries focused on the period of 18:01 to 00:00 (60 cases,78.94％),and limbs were the main injury parts(73 cases,96.05％).According to Downey's system,patients of grade 0 were 3 cases (3.95 ％),grade Ⅰ were 9 cases (11.84％),grade Ⅱ were 26 cases (34.21％),grade Ⅲ were 30 cases (39.47 ％),and grade Ⅳ were 8 cases (10.53 ％).All children victims were given comprehensive and symptomatic treatment,in which 74 cases were clinically cured and 2 cases were disabled,but no deaths.There was positive correlation between the traditional classification method and Downey classification method (r=0.85,P ＜ 0.05),and there was also positive correlation between treatment time and length of hospital stay (r =0.91,P ＜ 0.05).There was positive correlation between Downey level and OCS,the occurrence of myocardial damage,treatment time and length of hospital stay(r =0.90,0.95,0.48,0.96,respectively,all P ＜ 0.05).Conclusions The diagnosis and treatment were more specified after using the Downey classification,snake venom poisoning in children is a medical emergency requiring immediate attention,early diagnosis and proper treatment can reduce its fatality and disability rate.